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　　原标题：小鼠模型鼻内给予益生菌鼠李糖乳杆菌GG可预防白桦花粉诱导的小过敏性哮喘
　　背景：益生菌在预防和治疗气道疾病（包括过敏）方面的靶向应用越来越受到关注。在此，我们研究了益生菌鼠李糖乳杆菌gg和鼠李糖乳杆菌gr-1预防性鼻内治疗过敏性哮喘小鼠模型的有益效果。
　　方法：在第1-4天和第8-11天，以5x108 cfu/剂量鼻内给药鼠李糖乳杆菌8次，然后在两周的哮喘诱导方案中交替给药白桦花粉提取物。根据血清抗体水平、支气管肺泡灌洗液细胞计数、肺组织学、肺细胞因子水平和气道高反应性分析预防性治疗的效果。通过鼻粘膜、粪便样本、颈部淋巴结和血液中细菌细胞计数来评估鼠李糖乳杆菌的定植和易位。荧光显微镜观察鼠李糖乳杆菌与固定的小鼠鼻粘膜细胞和气道巨噬细胞的结合。
　　结果：鼠李糖乳杆菌（L.rhamnosusGG）短暂定植小鼠上呼吸道，其定植率约为鼠李糖乳杆菌（L.rhamnosusGR-1）的10倍。可见荧光鼠李糖乳杆菌（L.rhamnosusGG）与小鼠鼻粘膜细胞和气道巨噬细胞的明显结合。用鼠李糖乳杆菌GG（而不是鼠李糖乳杆菌GR-1）进行预防性治疗后，支气管肺泡灌洗嗜酸细胞计数、肺白细胞介素-13和白细胞介素-5水平以及气道高反应性显著降低。同样观察到血清bet v 1-特异性Igg1下降的趋势。
　　结论：鼻内给予鼠李糖乳杆菌GG可以以一种特定的方式阻止桦树花粉引起的过敏性哮喘的主要特征的发展。


延伸阅读
Allergy                                                     
Intranasal administration of probiotic Lactobacillus rhamnosus GG prevents birch pollen-induced allergic asthma in a murine model 
DOI: 10.1111/all.13502
Abstract:
Background: There is an increasing interest in targeted application of probiotic bacteria for prevention and treatment of airway diseases, including allergies. Here, we investigated the beneficial effects of preventive intranasal treatment with probiotics Lactobacillus rhamnosus GG and L. rhamnosus GR-1 in a mouse model of allergic asthma.
Methods: L. rhamnosus was administered intranasally eight times on days 1-4 and 8-11 at 5x108 CFU/dose, followed by a two-week asthma induction protocol with birch pollen extract on alternating days. Effects of preventive treatment were analyzed based on serum antibody levels, bronchoalveolar lavage cell counts, lung histology, lung cytokine levels and airway hyperreactivity. Colonization and translocation of L. rhamnosus was assessed by bacterial cell counts in nasal mucosa, fecal samples, cervical lymph nodes and blood. Binding of fluorescent L. rhamnosus to fixed murine nasal mucosal cells and airway macrophages was visualized by fluorescence microscopy.
Results: Transient colonization of the murine upper airways by L. rhamnosus GG was demonstrated and was approximately ten times higher compared to L. rhamnosus GR-1.Marked binding of fluorescent L. rhamnosus GG to murine nasal mucosal cells and airway macrophages was visualized. Preventive treatment with L. rhamnosus GG (but not L.rhamnosus GR-1) resulted in a significant decrease in bronchoalveolar lavage eosinophil counts, lung interleukin-13 and interleukin-5 levels, and airway hyperreactivity. A tendency towards a decrease in serum Bet v 1-specific IgG1was likewise observed.
Conclusion: Intranasally administered L. rhamnosus GG prevents the development of cardinal features of birch pollen-induced allergic asthma in a strain-specific manner.
First Author:
Irina Spacova
Correspondence:
University of Antwerp, Department of Bioscience Engineering,Groenenborgerlaan 171, G.V.521, B-2020 Antwerp, Belgium
All Authors:
Irina Spacova, Mariya I. Petrova, Astrid Fremau, Lore Pollaris, JeroenVanoirbeek, Jan L. Ceuppens, Sven Seys, Sarah Lebeer
　　创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。